PMM2/Phosphomannomutase 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00351

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PMM2/Phosphomannomutase 2 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human PMM2/Phosphomannomutase 2 (Accession#: O15305; Met1-Ser246)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PMM2/Phosphomannomutase 2 Antibody

Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351]

Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351]

Western Blot: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - Anti-PMM2/Phosphomannomutase 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa (We are unsure as to the identity of these extra bands).
Immunohistochemistry-Paraffin: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351]

Immunohistochemistry-Paraffin: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351]

Immunohistochemistry-Paraffin: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - Immunochemical staining of human PMM2/Phosphomannomutase 2 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351]

Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351]

Immunoprecipitation: PMM2/Phosphomannomutase 2 Antibody [NBP3-00351] - PMM2/Phosphomannomutase 2 was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-PMM2/Phosphomannomutase 2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PMM2/Phosphomannomutase 2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa

Applications for PMM2/Phosphomannomutase 2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PMM2/Phosphomannomutase 2

PMM2, also known as Phosphomannomutase 2, is a 246 amino acid protein that is 28 kDa, catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Studies are being performed on the relationship of this protein to congenital disorder of glycosylation, hydrops fetalis, premature ovarian failure, cerebellar hypoplasia, metabolic disorders, alcohol abuse, intellectual disability, hypertrophic cardiomyopathy, cerebellar ataxia, galactosemia, peripheral neuropathy, hypogonadism, neuropathy, hypotonia, cardiomyopathy, thrombocytopenia, ataxia, alcoholism, and malaria. The PMM2 protein has also shown an interaction with HIST1H4A, HIST1H4B, HIST1H4D, HIST1H4E, HIST1H4C, and over 130 other proteins in synthesis of substrates in N-glycan biosynthesis, asparagine N-linked glycosylation, metabolism of proteins, post-translational protein modification, biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein, fructose and mannose metabolism, amino sugar and nucleotide sugar metabolism, GDP-mannose biosynthesis, and colanic acid building blocks biosynthesis pathways.

Alternate Names

CDG1, CDG1A, CDGS, EC 5.4.2.8, phosphomannomutase 2, PMM 2

Gene Symbol

PMM2

Additional PMM2/Phosphomannomutase 2 Products

Product Documents for PMM2/Phosphomannomutase 2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PMM2/Phosphomannomutase 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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