PNK Antibody

Immunohistochemistry-Paraffin: PNK Antibody [NBP1-87257]

Immunohistochemistry-Paraffin: PNK Antibody [NBP1-87257] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Simple Western: PNK Antibody [NBP1-87257]

Simple Western: PNK Antibody [NBP1-87257] - Simple Western lane view shows a specific band for PNK in 0.5 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: PNK Antibody [NBP1-87257]

Simple Western: PNK Antibody [NBP1-87257] - Electropherogram image(s) of corresponding Simple Western lane view. PNK antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Western Blot: PNK Antibody [NBP1-87257] -

Western Blot: PNK Antibody [NBP1-87257] - Lysine 138, arginine 139 & arginine 141 are important for efficient DNA repair.(A) PNKP depletion by 3’-UTR siRNA PNKP was confirmed by western blot analysis. KAP1 antibody was used for loading control. (B) DNA DSB repair efficiency was analyzed by immunofluorescence using gamma-H2AX antibody after IR exposure in GFP-PNKP & 3’-UTR siRNA PNKP-transfected U2OS cells. Graph represented percentage of gamma-H2AX positive cells with 10 ≧ gamma-H2AX foci. At least 200 cells were counted & experiments were performed 3 times independently. (C) DNA SSB repair efficiency was analyzed by immunofluorescence using PAN-ADP-ribose binding reagents at 30 mins after IR exposure in GFP-PNKP & 3’-UTR siRNA PNKP-transfected U2OS cells treated with 10μM of poly (ADP-ribose) glycohydrolase inhibitor (PARGi) for 30 mins prior to IR exposure. (D) Graph represented the intensity of ADP ribose. At least 100 cells were counted & experiments were performed 3 times independently. Error bars indicate the standard error of the mean (SEM). ns: not significant (p > 0.05); **: 0.005 < p ≦ 0.01; ****: 0.0005 < p ≦ 0.001. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32970693), licensed under a CC-BY license. Not internally tested by Novus Biologicals.