PNK Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12588
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human PNK fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for PNK Antibody
![Western Blot: PNK Antibody [NBP3-12588]](https://resources.bio-techne.com/images/products/PNK-Antibody-Western-Blot-NBP3-12588-img0001.jpg "Western Blot: PNK Antibody [NBP3-12588]")
Western Blot: PNK Antibody [NBP3-12588]
Western Blot: PNK Antibody [NBP3-12588] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: PC-3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.)![Immunoprecipitation: PNK Antibody [NBP3-12588]](https://resources.bio-techne.com/images/products/PNK-Antibody-Immunoprecipitation-NBP3-12588-img0002.jpg "Immunoprecipitation: PNK Antibody [NBP3-12588]")
Immunoprecipitation: PNK Antibody [NBP3-12588]
Immunoprecipitation: PNK Antibody [NBP3-12588] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PNKP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PNKP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :60 kDaApplications for PNK Antibody
Application
Recommended Usage
Immunoprecipitation
1-5uL/mg of uLysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PNK
Long Name
Bifunctional polynucleotide phosphatase/kinase
Alternate Names
PNKP
Gene Symbol
PNKP
Additional PNK Products
Product Specific Notices for PNK Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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