PNPO Antibody (2C7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97941
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2C7
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human PNPO (57-261aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for PNPO Antibody (2C7) - BSA Free
Western Blot: PNPO Antibody (2C7)BSA Free [NBP1-97941]
Western Blot: PNPO Antibody (2C7) [NBP1-97941] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanPNPO antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: A549 cell lysateLane 2.: HepG2 cell lysateImmunocytochemistry/ Immunofluorescence: PNPO Antibody (2C7) - BSA Free [NBP1-97941]
Immunocytochemistry/Immunofluorescence: PNPO Antibody (2C7) [NBP1-97941] - Analysis of PNPO in Hep3B cells. The cell was stainedwith ATGA0213 (1:100). The secondary antibody (green) wasused Alexa Fluor 488. DAPI was stained the cell nucleus(blue).Immunohistochemistry-Paraffin: PNPO Antibody (2C7) - BSA Free [NBP1-97941]
Immunohistochemistry-Paraffin: PNPO Antibody (2C7) [NBP1-97941] - Paraffin embedded sections of human breast cancertissue were incubated with anti-human PNPO (1:150) for2 hours at room temperature. Antigen retrieval wasperformed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine(DAB).Applications for PNPO Antibody (2C7) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PNPO
Alternate Names
EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase
Gene Symbol
PNPO
UniProt
Additional PNPO Products
Product Documents for PNPO Antibody (2C7) - BSA Free
Product Specific Notices for PNPO Antibody (2C7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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