PNPO Antibody (2C7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97941

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C7

Format

BSA Free

Concentration

1 mg/ml

View all PNPO Antibodies »

Product Summary for PNPO Antibody (2C7) - BSA Free

Immunogen

Recombinant human PNPO (57-261aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PNPO Antibody (2C7) - BSA Free

Western Blot: PNPO Antibody (2C7)BSA Free [NBP1-97941]

Western Blot: PNPO Antibody (2C7)BSA Free [NBP1-97941]

Western Blot: PNPO Antibody (2C7) [NBP1-97941] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanPNPO antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1.: A549 cell lysateLane 2.: HepG2 cell lysate
Immunocytochemistry/ Immunofluorescence: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunocytochemistry/ Immunofluorescence: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunocytochemistry/Immunofluorescence: PNPO Antibody (2C7) [NBP1-97941] - Analysis of PNPO in Hep3B cells. The cell was stainedwith ATGA0213 (1:100). The secondary antibody (green) wasused Alexa Fluor 488. DAPI was stained the cell nucleus(blue).
Immunohistochemistry-Paraffin: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunohistochemistry-Paraffin: PNPO Antibody (2C7) - BSA Free [NBP1-97941]

Immunohistochemistry-Paraffin: PNPO Antibody (2C7) [NBP1-97941] - Paraffin embedded sections of human breast cancertissue were incubated with anti-human PNPO (1:150) for2 hours at room temperature. Antigen retrieval wasperformed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine(DAB).
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Applications for PNPO Antibody (2C7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNPO

PNPO (pyridoxamine 5'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.

Alternate Names

EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase

Gene Symbol

PNPO

UniProt

NP_060599 (Human)

Additional PNPO Products

Product Documents for PNPO Antibody (2C7) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PNPO Antibody (2C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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