PNPO Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87303

Novus Biologicals, part of Bio-Techne
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NBP1-87303
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PNPO Antibodies »

Product Summary for PNPO Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PNPO Antibody

Western Blot: PNPO Antibody [NBP1-87303]

Western Blot: PNPO Antibody [NBP1-87303]

Western Blot: PNPO Antibody [NBP1-87303] - Analysis using Anti-PNPO antibody NBP1-87303 (A) shows similar pattern to independent antibody NBP1-87302 (B).
Immunocytochemistry/ Immunofluorescence: PNPO Antibody [NBP1-87303]

Immunocytochemistry/ Immunofluorescence: PNPO Antibody [NBP1-87303]

Immunocytochemistry/Immunofluorescence: PNPO Antibody [NBP1-87303] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: PNPO Antibody [NBP1-87303]

Immunohistochemistry-Paraffin: PNPO Antibody [NBP1-87303]

Immunohistochemistry-Paraffin: PNPO Antibody [NBP1-87303] - Staining of human colon.
View 5 more images

Applications for PNPO Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization, Use PFA/Triton X-100. Western Blot verified by a customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNPO

Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. The rate-limiting enzyme in vitamin B6 synthesis is pyridoxine-5-prime-phosphate (PNP) oxidase (PNPO; EC 1.4.3.5).[supplied by OMIM]

Alternate Names

EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase

Gene Symbol

PNPO

Additional PNPO Products

Product Documents for PNPO Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PNPO Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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