PNPO Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35861
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PNPO (NP_060599.1).
Sequence:
MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP
Sequence:
MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PNPO Antibody
Western Blot: PNPO Antibody [NBP3-35861] -
Western Blot: PNPO Antibody [NBP3-35861] - Western blot analysis of various lysates using PNPO Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: PNPO Antibody [NBP3-35861] -
Immunocytochemistry/ Immunofluorescence: PNPO Antibody [NBP3-35861] - Immunofluorescence analysis of L929 cells using PNPO Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: PNPO Antibody [NBP3-35861] -
Immunocytochemistry/ Immunofluorescence: PNPO Antibody [NBP3-35861] - Immunofluorescence analysis of U2OS cells using PNPO Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for PNPO Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PNPO
Alternate Names
EC 1.4.3.5, FLJ10535, PDXPO, pyridoxal 5'-phosphate synthase, pyridoxamine 5'-phosphate oxidase, Pyridoxamine-phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine-5'-phosphate oxidase
Gene Symbol
PNPO
Additional PNPO Products
Product Documents for PNPO Antibody
Product Specific Notices for PNPO Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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