PNPT1 Antibody (4C11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43725
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4C11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to human PNPT1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PNPT1 Antibody (4C11)
![Western Blot: PNPT1 Antibody (4C11) [NBP2-43725]](https://resources.bio-techne.com/images/products/PNPT1-Antibody-4C11-Western-Blot-NBP2-43725-img0002.jpg "Western Blot: PNPT1 Antibody (4C11) [NBP2-43725]")
Western Blot: PNPT1 Antibody (4C11) [NBP2-43725]
Western Blot: PNPT1 Antibody (4C11) [NBP2-43725] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PNPase antibody [4C11] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
Immunocytochemistry/ Immunofluorescence: PNPT1 Antibody (4C11) [NBP2-43725] -
Immunocytochemistry/ Immunofluorescence: PNPT1 Antibody (4C11) [NBP2-43725] - PNPT1 antibody [4C11] detects PNPT1 protein at cell junction by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PNPT1 stained by PNPT1 antibody [4C11] (NBP2-43725) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10um.Applications for PNPT1 Antibody (4C11)
Application
Recommended Usage
Western Blot
1:500-1:3000
Application Notes
IP-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH7), 20% Glycerol
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PNPT1
Alternate Names
DKFZp762K1914, EC 2.7.7, EC 2.7.7.8, mitochondrial, OLD35, old-35, PNPase, PNPase 1, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, polyribonucleotide nucleotidyltransferase 1
Gene Symbol
PNPT1
Additional PNPT1 Products
Product Documents for PNPT1 Antibody (4C11)
Product Specific Notices for PNPT1 Antibody (4C11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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