Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PNPT1 Antibodies »

Product Summary for PNPT1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 700 and 750 of human Polynucleotide Phosphorylase 1 using the numbering given in entry NP_149100.2 (GeneID 87178).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PNPT1 Antibody

Western Blot: PNPT1 Antibody [NBP2-22269]

Western Blot: PNPT1 Antibody [NBP2-22269]

Western Blot: PNPT1 Antibody [NBP2-22269] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22269 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: PNPT1 Antibody [NBP2-22269]

Immunoprecipitation: PNPT1 Antibody [NBP2-22269]

Immunoprecipitation: PNPT1 Antibody [NBP2-22269] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22269 used for IP at 6 ug/mg lysate. PNPT1 was also immunoprecipitated by rabbit anti-PNPT1 antibody. For blotting immunoprecipitated PNPT1, NBP2-22269 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PNPT1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PNPT1

PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM]

Alternate Names

DKFZp762K1914, EC 2.7.7, EC 2.7.7.8, mitochondrial, OLD35, old-35, PNPase, PNPase 1, PNPase old-35, Polynucleotide phosphorylase 1, Polynucleotide phosphorylase-like protein, polyribonucleotide nucleotidyltransferase 1

Gene Symbol

PNPT1

UniProt

NP_149100.2 (Human)

Additional PNPT1 Products

Product Documents for PNPT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PNPT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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