Podocalyxin Like Antibody (6S9A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16434

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16434-100ul
NBP3-16434-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6S9A9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-479 of human Podocalyxin Like (O00592). SPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAEDRFSMPLIITIVCMASFLLLVAAL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Podocalyxin Like Antibody (6S9A9)

Western Blot: Podocalyxin Like Antibody (6S9A9) [NBP3-16434]

Western Blot: Podocalyxin Like Antibody (6S9A9) [NBP3-16434]

Western Blot: Podocalyxin Like Antibody (6S9A9) [NBP3-16434] - Western blot analysis of extracts of various cell lines, using Podocalyxin Like antibody (NBP3-16434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (6S9A9) [NBP3-16434]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (6S9A9) [NBP3-16434]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (6S9A9) [NBP3-16434] - Immunohistochemistry of paraffin-embedded human kidney cancer using Podocalyxin Like Rabbit mAb (NBP3-16434) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Podocalyxin Like Antibody (6S9A9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (6S9A9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Podocalyxin Like Antibody (6S9A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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