Podocalyxin Like Antibody (CL0284)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52903

Novus Biologicals, part of Bio-Techne
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NBP2-52903
NBP2-52903-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0284

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGD

Epitope

GASDEKLISLICRAV

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Podocalyxin Like Antibody (CL0284)

Western Blot: Podocalyxin Like Antibody (CL0284) [NBP2-52903]

Western Blot: Podocalyxin Like Antibody (CL0284) [NBP2-52903]

Western Blot: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Lane 1: Marker [kDa], Lane 2: Human kidney tissue lysate
Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (CL0284) [NBP2-52903]

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody (CL0284) [NBP2-52903]

Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Staining of U-251 cells using the Anti-PODXL monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (CL0284) [NBP2-52903] - Staining in human kidney and liver tissues. Corresponding PODXL RNA-seq data are presented for the same tissues.
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Applications for Podocalyxin Like Antibody (CL0284)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (CL0284)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Podocalyxin Like Antibody (CL0284)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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