Podocalyxin Like Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-41503

Novus Biologicals, part of Bio-Techne
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NB110-41503
NB110-41503SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.90 mg/ml

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

A synthetic peptide within an internal region (residues 100-200) of the human Podocalyxin protein. This immunogen should be far enough away from any glycosylation site.

Localization

Membrane

Marker

Podocytes /Apical Marker, Endothelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Podocalyxin Like Antibody

Western Blot: Podocalyxin Like Antibody [NB110-41503]

Western Blot: Podocalyxin Like Antibody [NB110-41503]

Western Blot: Podocalyxin Like Antibody [NB110-41503] - Analysis of Podolyxin in 1. Human thymus and 2. Human lymph node.
Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NB110-41503]

Immunocytochemistry/ Immunofluorescence: Podocalyxin Like Antibody [NB110-41503]

Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NB110-41503] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Podocalyxin Like Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for Podocalyxin Like Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is observed at ~52 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC/IF membrane staining was observed.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NB110-41503 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine, 0.15 M NaCl

Preservative

0.05% Sodium Azide

Concentration

0.90 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Podocalyxin

Podocalyxin (PODXL) is an extensively N/O-glycosylated and sialylated type I transmembrane protein that contains C-terminal PDZ-binding motif DTHL capable of facilitating interactions with NHERF1/NHERF2 adaptor proteins which complexes with proteins implicated in ion transport, protein trafficking and signal transduction. PODXL function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in podocytes by charge repulsion. PODXL also acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. It induces apical actin-dependent microvilli formation and is implicated preapical plasma membrane subdomain formation to set up inital epithelial polarization and apical lumen formation during renal tubulogenesis. PODXL is highly expressed in renal glomeruli, embryonic germ layers, hematopoietic progenitors, megakaryocytes and platelets, vascular endothelia, mesothelial cells lining organs, and neurons. In several malignancies such as leukemia and cancer of breast, testicular, prostate etc, PODXL interactions with actin-binding protein EZR enhances cancer aggressiveness by inducing cell migration and invasion through via modulation of MAPK/PI3K signaling.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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