Podoplanin Antibody (PDPN/8875R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23708
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # PDPN/8875R
Concentration
0.2 mg/ml
Product Summary for Podoplanin Antibody (PDPN/8875R)
Immunogen
Recombinant fragment (around aa1-150) of human Podoplanin protein (exact sequence is proprietary)
Localization
Cell surface.
Marker
Lymphatic Endothelial & Mesothelial Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for Podoplanin Antibody (PDPN/8875R)
Immunohistochemistry-Paraffin: Podoplanin Antibody (PDPN/8875R) [NBP3-23708] -
Formalin-fixed, paraffin-embedded human prostate stained with Podoplanin antibody (PDPN/8875R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Podoplanin Antibody (PDPN/8875R)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Podoplanin
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Alternate Names
Aggrus, Gp38, PDPN, RANDAM-2, T1A-2
Gene Symbol
PDPN
Additional Podoplanin Products
Product Documents for Podoplanin Antibody (PDPN/8875R)
Product Specific Notices for Podoplanin Antibody (PDPN/8875R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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