Podoplanin Antibody (pmab-1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03955

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # pmab-1

Format

BSA Free

Concentration

1.0 mg/ml

View all Podoplanin Antibodies »

Product Specifications

Immunogen

Podoplanin

Marker

Lymphatic Endothelium Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Podoplanin Antibody (pmab-1) - BSA Free

Western Blot: Podoplanin Antibody (pmab-1)BSA Free [NBP2-03955]

Western Blot: Podoplanin Antibody (pmab-1)BSA Free [NBP2-03955]

Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Lysate from mouse A) Brain, B) Heart, C) Small Intestine, D) Kidney, E) Liver, F) Lung, G) Muscle, H) Stomach, I) Spleen, J) Ovary, and K) Testis probed with podoplanin antibody at 5 ug/mL. Mouse tissue Insta-blot, goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: Podoplanin Antibody (pmab-1) - BSA Free [NBP2-03955]

Flow Cytometry: Podoplanin Antibody (pmab-1) - BSA Free [NBP2-03955]

Flow Cytometry: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the PE conjugate of NBP2-03955. Staining using podoplanin antibody. Mouse F9 tumor cell line probed using 0.5 ug of podoplanin antibody (red) and 0.5 ug of isotype control antibody (green). Cell surface flow kit was used for this test.
Western Blot: Podoplanin Antibody (pmab-1)BSA Free [NBP2-03955]

Western Blot: Podoplanin Antibody (pmab-1)BSA Free [NBP2-03955]

Western Blot: Podoplanin Antibody (pmab-1) [NBP2-03955] - Analysis using the Azide Free version of NBP2-03955. Detection of mouse fetal lung tissue using Podoplanin antibody at a conentration of 1 ug/mL.
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Applications for Podoplanin Antibody (pmab-1) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 ug/10^5 cells

Western Blot

3 - 5 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Podoplanin

Podoplanin, also known as gp38, is a 43kD type 1 transmembrane glycoprotein found on many tissues. Podoplanin appears to be differentially expressed by lymphatic endothelial cells but not blood vessel endothelial cells (Schacht, 2005). Podoplanin has also been found to be expressed on a wide variety of tumors. It appears to be involved in lymphangioigenesis and promoting cell migration (Farr, 1992). Recently, there are indications of podoplanin presence on Th17 cells (Bettelli, 2011). In a murine EAE model for autoimmune disease it has been shown that Th17 cells migrate quickly to the CNS at EAE onset. Injection of antibody to podoplanin in mice also immunized with myelin antigen to induce EAE results in elevated numbers of Th17 cells in the CNS. Thus as in effecting migration of tumor cells, podoplanin may also promote migration of committed Th17 cells to sites of inflammation in autoimmune diseases.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Alternate Names

Aggrus, Gp38, PDPN, RANDAM-2, T1A-2

Gene Symbol

PDPN

UniProt

NP_034459.2 (Mouse)

Additional Podoplanin Products

Product Documents for Podoplanin Antibody (pmab-1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Podoplanin Antibody (pmab-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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