Podoplanin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1014
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human Podoplanin
Localization
Type I membrane protein.
Marker
Lymphatic Endothelium Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Podoplanin Antibody
![Western Blot: Podoplanin Antibody [NB600-1014]](https://resources.bio-techne.com/images/products/Podoplanin-Antibody-Western-Blot-NB600-1014-img0001.jpg "Western Blot: Podoplanin Antibody [NB600-1014]")
Western Blot: Podoplanin Antibody [NB600-1014]
Western Blot: Podoplanin Antibody [NB600-1014] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.Applications for Podoplanin Antibody
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunoprecipitation
1:10-1:500
Western Blot
1-5 ug/ml
Application Notes
WB: Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry reactivity reported in (PMID:21406228).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Podoplanin
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Alternate Names
Aggrus, Gp38, PDPN, RANDAM-2, T1A-2
Entrez Gene IDs
10630 (Human)
Gene Symbol
PDPN
Additional Podoplanin Products
Product Documents for Podoplanin Antibody
Product Specific Notices for Podoplanin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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