POLA2 Antibody (2F11)
Novus Biologicals, part of Bio-Techne | Catalog # H00023649-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2F11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLA2 (AAH01347, 1 a.a. ~ 598 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSASAQQLAEELQIFGLDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDREHSSGAQIPVDLSELKEYSLFPGQVVIMEGINTTGRKLVATKLYEGVPLPFYQPTEEDADFEQSMVLVACGPYTTSDSITYDPLLDLIAVINHDRPDVCILFGPFLDAKHEQVENCLLTSPFEDIFKQCLRTIIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFSYSDLSREDKKQVQFVSEPCSLSINGVIFGLTSTDLLFHLGAEEISSSSGTSDRFSRILKHILTQRSYYPLYPPQEDMAIDYESFYVYAQLPVTPDVLIIPSELRYFVKDVLGCVCVNPGRLTKGQVGGTFARLYLRRPAADGAERQSPCIAVQVVRI
Specificity
POLA2 - polymerase (DNA directed), alpha 2 (70kD subunit)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for POLA2 Antibody (2F11)
![Western Blot: POLA2 Antibody (2F11) [H00023649-M01]](https://resources.bio-techne.com/images/products/POLA2-Antibody-2F11-Western-Blot-H00023649-M01-img0007.jpg "Western Blot: POLA2 Antibody (2F11) [H00023649-M01]")
Western Blot: POLA2 Antibody (2F11) [H00023649-M01]
Western Blot: POLA2 Antibody (2F11) [H00023649-M01] - POLA2 monoclonal antibody (M01), clone 2F11 Analysis of POLA2 expression in Hela.![Immunocytochemistry/ Immunofluorescence: POLA2 Antibody (2F11) [H00023649-M01]](https://resources.bio-techne.com/images/products/POLA2-Antibody-2F11-Immunocytochemistry-Immunofluorescence-H00023649-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: POLA2 Antibody (2F11) [H00023649-M01]")
Immunocytochemistry/ Immunofluorescence: POLA2 Antibody (2F11) [H00023649-M01]
Immunocytochemistry/Immunofluorescence: POLA2 Antibody (2F11) [H00023649-M01] - Analysis of monoclonal antibody to POLA2 on HeLa cell. Antibody concentration 10 ug/ml.![ELISA: POLA2 Antibody (2F11) [H00023649-M01]](https://resources.bio-techne.com/images/products/POLA2-Antibody-2F11-ELISA-H00023649-M01-img0008.jpg "ELISA: POLA2 Antibody (2F11) [H00023649-M01]")
ELISA: POLA2 Antibody (2F11) [H00023649-M01]
ELISA: POLA2 Antibody (2F11) [H00023649-M01] - Detection limit for recombinant GST tagged POLA2 is approximately 0.1ng/ml as a capture antibody.Applications for POLA2 Antibody (2F11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: POLA2
Long Name
DNA polymerase alpha subunit B
Alternate Names
DNA polymerase alpha 70 kDa subunit, DNA polymerase alpha subunit B, FLJ21662, FLJ37250, polymerase (DNA directed), alpha 2 (70kD subunit), polymerase (DNA-directed), alpha (70kD)
Entrez Gene IDs
23649 (Human)
Gene Symbol
POLA2
UniProt
Additional POLA2 Products
Product Documents for POLA2 Antibody (2F11)
Product Specific Notices for POLA2 Antibody (2F11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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