POLA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82139

Novus Biologicals, part of Bio-Techne
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NBP1-82139
NBP1-82139-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLA2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IMEGINTTGRKLVATKLYEGVPLPFYQPTEEDADFEQSMVLVACGPYTTSDSITYDPLLDLIAVINHDRPDVCILFGPFLDAKHEQVENCL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLA2 Antibody

Immunohistochemistry-Paraffin: POLA2 Antibody [NBP1-82139]

Immunohistochemistry-Paraffin: POLA2 Antibody [NBP1-82139]

Immunohistochemistry-Paraffin: POLA2 Antibody [NBP1-82139] - Staining in human lymph node and liver tissues using anti-POLA2 antibody. Corresponding POLA2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: POLA2 Antibody [NBP1-82139]

Immunocytochemistry/ Immunofluorescence: POLA2 Antibody [NBP1-82139]

Immunocytochemistry/Immunofluorescence: POLA2 Antibody [NBP1-82139] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: POLA2 Antibody [NBP1-82139]

Immunohistochemistry-Paraffin: POLA2 Antibody [NBP1-82139]

Immunohistochemistry-Paraffin: POLA2 Antibody [NBP1-82139] - Staining of human lymph node shows high expression.
View 1 more images

Applications for POLA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLA2

POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery

Long Name

DNA polymerase alpha subunit B

Alternate Names

DNA polymerase alpha 70 kDa subunit, DNA polymerase alpha subunit B, FLJ21662, FLJ37250, polymerase (DNA directed), alpha 2 (70kD subunit), polymerase (DNA-directed), alpha (70kD)

Gene Symbol

POLA2

Additional POLA2 Products

Product Documents for POLA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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