POLDIP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68874

Novus Biologicals, part of Bio-Techne
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NBP1-68874
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all POLDIP2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence KTHTYYQVLIDARDC corresponding to internal region according to NP_056399.1.

Epitope

KTHTYYQVLIDARDC

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for POLDIP2 Antibody

Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874] - Staining of K562 (A), MCF7 (B) and Mouse Liver (C) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C. Detected by chemiluminescence.
Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874]

Western Blot: POLDIP2 Antibody [NBP1-68874] - (1ug/ml) Analysis of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for POLDIP2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in lysates of K562 and MCF7 and of mouse liver (calculated MW of 42.0 kDa band according to NP_056399.1). Approx. 37 kDa band observed in rat vascular smooth muscle cells lysates. The band dimished upon siRNA knock down.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLDIP2

This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. The encoded protein also interacts with proliferating cell nuclear antigen. Some transcripts of this gene overlap in a tail-to-tail orientation with the gene for tumor necrosis factor, alpha-induced protein 1 (TNFAIP1).

Alternate Names

DKFZP586F1524, p38, PDIP38DKFZp586F1524,38 kDa DNA polymerase delta interaction protein, POLD4, polymerase (DNA-directed), delta interacting protein 2, polymerase delta interacting protein 38, polymerase delta-interacting protein 2

Entrez Gene IDs

26073 (Human); 67811 (Mouse)

Gene Symbol

POLDIP2

UniProt

NP_056399.1 (Human)

Additional POLDIP2 Products

Product Documents for POLDIP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLDIP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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