POLG2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94064

Novus Biologicals, part of Bio-Techne
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NBP2-94064-0.02ml
NBP2-94064-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all POLG2 Antibodies »

Product Summary for POLG2 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2). HPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLG2 Antibody - BSA Free

Western Blot: POLG2 AntibodyBSA Free [NBP2-94064]

Western Blot: POLG2 AntibodyBSA Free [NBP2-94064]

Western Blot: POLG2 Antibody [NBP2-94064] - Analysis of extracts of various cell lines, using POLG2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: POLG2 Antibody - BSA Free [NBP2-94064]

Immunocytochemistry/ Immunofluorescence: POLG2 Antibody - BSA Free [NBP2-94064]

Immunocytochemistry/Immunofluorescence: POLG2 Antibody [NBP2-94064] - Analysis of MCF7 cells using POLG2 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: POLG2 Antibody - BSA Free [NBP2-94064]

Immunohistochemistry-Paraffin: POLG2 Antibody - BSA Free [NBP2-94064]

Immunohistochemistry-Paraffin: POLG2 Antibody [NBP2-94064] - Rat heart using POLG2 at dilution of 1:100 (40x lens).
View 3 more images

Applications for POLG2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLG2

The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit (POLG1; MIM 174763) and a 55-kD accessory subunit (POLG2).[supplied by OMIM]

Alternate Names

DNA polymerase gamma accessory 55 kDa subunit, DNA polymerase subunit gamma-2, mitochondrial, EC 2.7.7.7, HP55, Mitochondrial DNA polymerase accessory subunit, mitochondrial DNA polymerase subunit gamma-2, mitochondrial DNA polymerase, accessory subunit, MtPolB, MTPOLBPOLG-BETA, p55, PEOA4, POLB, POLGB, PolG-beta, polymerase (DNA directed), gamma 2, accessory subunit

Gene Symbol

POLG2

Additional POLG2 Products

Product Documents for POLG2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLG2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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