POLR1B Antibody (4H6)

Novus Biologicals, part of Bio-Techne | Catalog # H00084172-M10

Novus Biologicals, part of Bio-Techne
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H00084172-M10
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR1B Antibodies »

Product Specifications

Immunogen

POLR1B (NP_061887, 963 a.a. ~ 1072 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVK

Specificity

POLR1B (4H6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for POLR1B Antibody (4H6)

Western Blot: POLR1B Antibody (4H6) [H00084172-M10]

Western Blot: POLR1B Antibody (4H6) [H00084172-M10]

Western Blot: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of POLR1B expression in transfected 293T cell line by POLR1B monoclonal antibody (M10), clone 4H6. Lane 1: POLR1B transfected lysate (Predicted MW: 128.2 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: POLR1B Antibody (4H6) [H00084172-M10]

Immunocytochemistry/ Immunofluorescence: POLR1B Antibody (4H6) [H00084172-M10]

Immunocytochemistry/Immunofluorescence: POLR1B Antibody (4H6) [H00084172-M10] - Analysis of monoclonal antibody to POLR1B on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: POLR1B Antibody (4H6) [H00084172-M10] - Detection limit for recombinant GST tagged POLR1B is 3 ng/ml as a capture antibody.

Applications for POLR1B Antibody (4H6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POLR1B

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM]

Alternate Names

DNA-directed RNA polymerase I 135 kDa polypeptide, DNA-directed RNA polymerase I 135kDa polypeptide, DNA-directed RNA polymerase I subunit RPA2, EC 2.7.7.6, FLJ10816, FLJ21921, MGC131780, polymerase (RNA) I polypeptide B, 128kDa, RNA polymerase I subunit 2, RPA116, RPA135, RPA2, Rpo1-2

Entrez Gene IDs

84172 (Human)

Gene Symbol

POLR1B

UniProt

NP_061887 (Human)

Additional POLR1B Products

Product Documents for POLR1B Antibody (4H6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POLR1B Antibody (4H6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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