POLR1C Antibody (M1)
Novus Biologicals, part of Bio-Techne | Catalog # H00009533-M05
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # M1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLR1C (AAH08863, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAASQAVEEMRSRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHDDILIAQLRPGQEIDLLMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSRCFSPGVIEVQEVQGKKVARVANPRLDTFSREIFRNEKLKKVVRLARVRDHYIFSVESTGVLPPDVLVSEAIKVLMGKCRRFLDELDAVQMD
Reactivity Notes
Human. Other species not tested.
Localization
Nuclear
Specificity
POLR1C - polymerase (RNA) I polypeptide C, 30kDa
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for POLR1C Antibody (M1)
![Western Blot: POLR1C Antibody (M1) [H00009533-M05]](https://resources.bio-techne.com/images/products/POLR1C-Antibody-M1-Western-Blot-H00009533-M05-img0007.jpg "Western Blot: POLR1C Antibody (M1) [H00009533-M05]")
Western Blot: POLR1C Antibody (M1) [H00009533-M05]
Western Blot: POLR1C Antibody (M1) [H00009533-M05] - Analysis of POLR1C expression in HeLa (Cat # L013V1).![Immunohistochemistry-Paraffin: POLR1C Antibody (M1) [H00009533-M05]](https://resources.bio-techne.com/images/products/POLR1C-Antibody-M1-Immunohistochemistry-Paraffin-H00009533-M05-img0005.jpg "Immunohistochemistry-Paraffin: POLR1C Antibody (M1) [H00009533-M05]")
Immunohistochemistry-Paraffin: POLR1C Antibody (M1) [H00009533-M05]
Immunohistochemistry-Paraffin: POLR1C Antibody (M1) [H00009533-M05] - Analysis of monoclonal antibody to POLR1C on formalin-fixed paraffin-embedded human pancreatic cancer. Antibody concentration 0.6 ug/ml
Sandwich ELISA: POLR1C Antibody (M1) [H00009533-M05] - Detection limit for recombinant GST tagged POLR1C is approximately 0.3ng/ml as a capture antibody.
Applications for POLR1C Antibody (M1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: POLR1C
Alternate Names
AC40, DNA-directed RNA polymerase I subunit C, DNA-directed RNA polymerases I and III 40 kDa polypeptide, POLR1E, polymerase (RNA) I polypeptide C, 30kDa, RNA polymerase I subunit, RPA39RNA polymerases I and III subunit AC1, RPA40TCS3, RPA5RPAC1DNA-directed RNA polymerases I and III subunit RPAC1, RPC40
Entrez Gene IDs
9533 (Human)
Gene Symbol
POLR1C
UniProt
Additional POLR1C Products
Product Documents for POLR1C Antibody (M1)
Product Specific Notices for POLR1C Antibody (M1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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