POLR2C Antibody (3T8K8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16871
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3T8K8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for POLR2C Antibody (3T8K8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POLR2C (P19387). MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for POLR2C Antibody (3T8K8)
Western Blot: POLR2C Antibody (3T8K8) [NBP3-16871]
Western Blot: POLR2C Antibody (3T8K8) [NBP3-16871] - Western blot analysis of extracts of various cell lines, using POLR2C antibody (NBP3-16871) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Immunohistochemistry-Paraffin: POLR2C Antibody (3T8K8) [NBP3-16871]
Immunohistochemistry-Paraffin: POLR2C Antibody (3T8K8) [NBP3-16871] - Immunohistochemistry of paraffin-embedded human lung cancer using POLR2C Rabbit mAb (NBP3-16871) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: POLR2C Antibody (3T8K8) [NBP3-16871]
Immunohistochemistry-Paraffin: POLR2C Antibody (3T8K8) [NBP3-16871] - Immunohistochemistry of paraffin-embedded rat lung using POLR2C Rabbit mAb (NBP3-16871) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for POLR2C Antibody (3T8K8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POLR2C
Alternate Names
DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, polymerase (RNA) II (DNA directed) polypeptide C (33kD), polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33
Gene Symbol
POLR2C
Additional POLR2C Products
Product Documents for POLR2C Antibody (3T8K8)
Product Specific Notices for POLR2C Antibody (3T8K8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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