POLR2C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80818

Novus Biologicals, part of Bio-Techne
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NBP1-80818
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR2C Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR2C Antibody

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818]

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818]

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP1-80818] - Staining of human cerebral cortex, colon, kidney and testis using Anti-POLR2C antibody NBP1-80818 (A) shows similar protein distribution across tissues to independent antibody NBP1-80817 (B).
Western Blot: POLR2C Antibody [NBP1-80818]

Western Blot: POLR2C Antibody [NBP1-80818]

Western Blot: POLR2C Antibody [NBP1-80818] - Analysis using Anti-POLR2C antibody NBP1-80818 (A) shows similar pattern to independent antibody NBP1-80817 (B).
Immunocytochemistry/ Immunofluorescence: POLR2C Antibody [NBP1-80818]

Immunocytochemistry/ Immunofluorescence: POLR2C Antibody [NBP1-80818]

Immunocytochemistry/Immunofluorescence: POLR2C Antibody [NBP1-80818] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
View 5 more images

Applications for POLR2C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR2C

The POLR2C gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit

Alternate Names

DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, polymerase (RNA) II (DNA directed) polypeptide C (33kD), polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33

Entrez Gene IDs

5432 (Human)

Gene Symbol

POLR2C

UniProt

P19387 (Human)

Additional POLR2C Products

Product Documents for POLR2C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR2C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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