POLR2C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19885

Novus Biologicals, part of Bio-Techne
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NBP2-19885
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (94%), Monkey (100%), Xenopus (94%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR2C Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human POLR2C. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (100%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2C Antibody

Western Blot: POLR2C Antibody [NBP2-19885]

Western Blot: POLR2C Antibody [NBP2-19885]

Western Blot: POLR2C Antibody [NBP2-19885] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: POLR2C Antibody [NBP2-19885]

Immunocytochemistry/ Immunofluorescence: POLR2C Antibody [NBP2-19885]

Immunocytochemistry/Immunofluorescence: POLR2C Antibody [NBP2-19885] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: POLR2C protein stained by POLR2C antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885]

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885]

Immunohistochemistry-Paraffin: POLR2C Antibody [NBP2-19885] - Paraffin-embedded mouse liver. POLR2C antibody [N1C3-2] dilution: 1:500.
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Applications for POLR2C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POLR2C

This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. [provided by RefSeq]

Alternate Names

DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, DNA-directed RNA polymerase II subunit RPB3, hRPB33, hsRPB3, polymerase (RNA) II (DNA directed) polypeptide C (33kD), polymerase (RNA) II (DNA directed) polypeptide C, 33kDa, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33

Gene Symbol

POLR2C

UniProt

P19387 (Human)

Additional POLR2C Products

Product Documents for POLR2C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POLR2C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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