POLR2D Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97517
![Western Blot: POLR2D Antibody [NBP2-97517]](https://resources.bio-techne.com/images/products/POLR2D-Antibody-Western-Blot-NBP2-97517-img0003.jpg "Western Blot: POLR2D Antibody [NBP2-97517]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for POLR2D Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human POLR2D fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for POLR2D Antibody
Western Blot: POLR2D Antibody [NBP2-97517]
Western Blot: POLR2D Antibody [NBP2-97517] - Anti-POLR2D rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: MCF-7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 18 kDa![Immunoprecipitation: POLR2D Antibody [NBP2-97517]](https://resources.bio-techne.com/images/products/POLR2D-Antibody-Immunoprecipitation-NBP2-97517-img0004.jpg "Immunoprecipitation: POLR2D Antibody [NBP2-97517]")
Immunoprecipitation: POLR2D Antibody [NBP2-97517]
Immunoprecipitation: POLR2D Antibody [NBP2-97517] - POLR2D was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-POLR2D rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-POLR2D rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 17 kDaApplications for POLR2D Antibody
Application
Recommended Usage
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POLR2D
Alternate Names
DNA-directed RNA polymerase II 16 kDa polypeptide, DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, polymerase (RNA) II (DNA directed) polypeptide D, RBP4, RNA polymerase II 16 kDa subunit, RNA polymerase II subunit B4, RNA polymerase II subunit hsRBP4, RPB16
Gene Symbol
POLR2D
Additional POLR2D Products
Product Specific Notices for POLR2D Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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