POLR2E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-57185

Novus Biologicals, part of Bio-Techne
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NBP2-57185
NBP2-57185-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR2E Antibodies »

Product Summary for POLR2E Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYFGIKRGQVVKIIRPSETAGRY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR2E Antibody

Western Blot: POLR2E Antibody [NBP2-57185]

Western Blot: POLR2E Antibody [NBP2-57185]

Western Blot: POLR2E Antibody [NBP2-57185] - Analysis using Anti-POLR2E antibody NBP2-57185 (A) shows similar pattern to independent antibody NBP2-49577 (B).
Immunocytochemistry/ Immunofluorescence: POLR2E Antibody [NBP2-57185]

Immunocytochemistry/ Immunofluorescence: POLR2E Antibody [NBP2-57185]

Immunocytochemistry/Immunofluorescence: POLR2E Antibody [NBP2-57185] - Staining of human cell line CACO-2 shows localization to nucleoplasm.

Applications for POLR2E Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR2E

POLR2E encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Alternate Names

DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1

Gene Symbol

POLR2E

Additional POLR2E Products

Product Documents for POLR2E Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR2E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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