POLR2F Antibody (2G2)
Novus Biologicals, part of Bio-Techne | Catalog # H00005435-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2G2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
POLR2F (NP_068809.1, 1 a.a. ~ 127 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD
Specificity
This product is specific for Human POLR2F monoclonal antibody (M02), clone 2G2 [Gene ID: 5435].
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for POLR2F Antibody (2G2)
![Western Blot: POLR2F Antibody (2G2) [H00005435-M02]](https://resources.bio-techne.com/images/products/POLR2F-Antibody-2G2-Western-Blot-H00005435-M02-img0004.jpg "Western Blot: POLR2F Antibody (2G2) [H00005435-M02]")
Western Blot: POLR2F Antibody (2G2) [H00005435-M02]
Western Blot: POLR2F Antibody (2G2) [H00005435-M02] - Analysis of POLR2F expression in transfected 293T cell line by POLR2F monoclonal antibody (M02), clone 2G2.Lane 1: POLR2F transfected lysate (Predicted MW: 14.5 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: POLR2F Antibody (2G2) [H00005435-M02]](https://resources.bio-techne.com/images/products/POLR2F-Antibody-2G2-Immunocytochemistry-Immunofluorescence-H00005435-M02-img0002.jpg "Immunocytochemistry/ Immunofluorescence: POLR2F Antibody (2G2) [H00005435-M02]")
Immunocytochemistry/ Immunofluorescence: POLR2F Antibody (2G2) [H00005435-M02]
Immunocytochemistry/Immunofluorescence: POLR2F Antibody (2G2) [H00005435-M02] - Analysis of monoclonal antibody to POLR2F on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: POLR2F Antibody (2G2) [H00005435-M02] - Detection limit for recombinant GST tagged POLR2F is 0.3 ng/ml as a capture antibody.
Applications for POLR2F Antibody (2G2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: POLR2F
Alternate Names
DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, HRBP14.4, II, and III subunit RPABC2, polymerase (RNA) II (DNA directed) polypeptide F, RNA Polymerase II subunit 14.4 kD, RNA polymerases I, II, and III subunit ABC2, RPB6, RPB6 homolog
Gene Symbol
POLR2F
UniProt
Additional POLR2F Products
Product Documents for POLR2F Antibody (2G2)
Product Specific Notices for POLR2F Antibody (2G2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...