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POLR2G Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47329

Novus Biologicals, part of Bio-Techne
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NBP2-47329
NBP2-47329-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR2G Antibody

Western Blot: POLR2G Antibody [NBP2-47329]

Western Blot: POLR2G Antibody [NBP2-47329]

Western Blot: POLR2G Antibody [NBP2-47329] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: POLR2G Antibody [NBP2-47329]

Immunocytochemistry/ Immunofluorescence: POLR2G Antibody [NBP2-47329]

Immunocytochemistry/Immunofluorescence: POLR2G Antibody [NBP2-47329] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: POLR2G Antibody [NBP2-47329]

Immunohistochemistry-Paraffin: POLR2G Antibody [NBP2-47329]

Immunohistochemistry-Paraffin: POLR2G Antibody [NBP2-47329] - Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.

Applications for POLR2G Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR2G

POLR2G encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, the association of this subunit with the polymerase under suboptimal growth conditions indicates it may play a role in regulating polymerase function.

Alternate Names

DNA directed RNA polymerase II 19 kda polypeptide, DNA-directed RNA polymerase II 19 kDa polypeptide, DNA-directed RNA polymerase II subunit G, DNA-directed RNA polymerase II subunit RPB7, hRPB19, hsRPB7, MGC138369, polymerase (RNA) II (DNA directed) polypeptide G, RNA polymerase II 19 kDa subunit, RNA polymerase II seventh subunit, RNA polymerase II subunit B7, RPB19, RPB7MGC138367

Gene Symbol

POLR2G

Additional POLR2G Products

Product Documents for POLR2G Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLR2G Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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