POLR3H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13787

Novus Biologicals, part of Bio-Techne
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NBP2-13787
NBP2-13787-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POLR3H Antibodies »

Product Summary for POLR3H Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: GFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for POLR3H Antibody

Western Blot: POLR3H Antibody [NBP2-13787]

Western Blot: POLR3H Antibody [NBP2-13787]

Western Blot: POLR3H Antibody [NBP2-13787] - Analysis in control (vector only transfected HEK293T lysate) and POLR3H over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: POLR3H Antibody [NBP2-13787]

Immunocytochemistry/ Immunofluorescence: POLR3H Antibody [NBP2-13787]

Immunocytochemistry/Immunofluorescence: POLR3H Antibody [NBP2-13787] - Staining of human cell line A-431 shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-13787]

Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-13787]

Immunohistochemistry-Paraffin: POLR3H Antibody [NBP2-13787] - Staining of human stomach, lower shows moderate nuclear positivity in glandular cells.

Applications for POLR3H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POLR3H

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Actsas nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves astemplate for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encodedRNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway

Alternate Names

DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide, DNA-directed RNA polymerase III subunit H, DNA-directed RNA polymerase III subunit RPC8, KIAA1665MGC29654, MGC111097, polymerase (RNA) III (DNA directed) polypeptide H (22.9kD), RNA nucleotidyltransferase (DNA-directed), RNA polymerase III subunit 22.9 kDa subunit, RNA polymerase III subunit C8, RNA polymerase III subunit RPC8, RPC8RPC22.9

Gene Symbol

POLR3H

Additional POLR3H Products

Product Documents for POLR3H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR3H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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