Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36523

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-36523
NBP2-36523-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HWA4C4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Poly-Ubiquitin Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing the ubiquitin K63-isopeptide motif.

Reactivity Notes

Does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species.

Specificity

Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage

Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]

Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]

Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.
Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523]

Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523]

Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Sections of hippocampus from human Alzheimers disease brain stained at 1:100 dilution with biotinylated secondary antibody and DAB detection.
Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]

Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]

Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis at 1:1000 dilution

Applications for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500 - 1:1000
Application Notes
Specificity is temperature sensitive. Western blot membranes must not be autoclaved. Excessive heat should be avoided in all applications.K63-linked polyubiquitin modified proteins comprise only a proportion of total cellular polyubiquitinylated species. Increased sample loading may therefore be required for analysis by Western blot. Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Poly-Ubiquitin

Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule. Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively. Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes.

Alternate Names

PolyUbiquitin

Gene Symbol

RPS27A

Additional Poly-Ubiquitin Products

Product Documents for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.