PON1 Antibody (4X7X2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16187

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16187-100ul
NBP3-16187-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4X7X2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PON1 Antibodies »

Product Summary for PON1 Antibody (4X7X2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PON1 (P27169). TGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PON1 Antibody (4X7X2)

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of Mouse liver, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: PON1 Antibody (4X7X2) [NBP3-16187]

Immunohistochemistry: PON1 Antibody (4X7X2) [NBP3-16187]

Immunohistochemistry: PON1 Antibody (4X7X2) [NBP3-16187] - Immunofluorescence analysis of mouse liver using PON1 Rabbit mAb (NBP3-16187) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187]

Western Blot: PON1 Antibody (4X7X2) [NBP3-16187] - Western blot analysis of extracts of HepG2 cells, using PON1 Rabbit mAb (NBP3-16187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
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Applications for PON1 Antibody (4X7X2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PON1

Paraoxonase 1 (PON1) is an arylesterase capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. PON1 primarily hydrolyzes paroxon, an organophosphorus anticholinesterase compound produced in vivo by oxidation of the insecticide parathion, to produce p-nitrophenol. Polymorphisms in this gene are a risk factor in coronary artery disease.

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Gene Symbol

PON1

Additional PON1 Products

Product Documents for PON1 Antibody (4X7X2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PON1 Antibody (4X7X2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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