PON1 Antibody (OTI2D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03706

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D4

Concentration

0.97 mg/ml

View all PON1 Antibodies »

Product Summary for PON1 Antibody (OTI2D4)

Immunogen

Full length human recombinant protein of human PON1 (NP_000437) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PON1 Antibody (OTI2D4)

Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706]

Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706]

Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.
Immunocytochemistry/ Immunofluorescence: PON1 Antibody (OTI2D4) [NBP2-03706]

Immunocytochemistry/ Immunofluorescence: PON1 Antibody (OTI2D4) [NBP2-03706]

Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.
Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706]

Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706]

Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.
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Applications for PON1 Antibody (OTI2D4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.97 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PON1

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq].

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Entrez Gene IDs

5444 (Human)

Gene Symbol

PON1

Additional PON1 Products

Product Documents for PON1 Antibody (OTI2D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PON1 Antibody (OTI2D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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