PON1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26401

Novus Biologicals, part of Bio-Techne
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NBP1-26401
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PON1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EEPKVTQVYAEN corresponding to internal region according to NP_000437.3.

Epitope

Internal region (EEPKVTQVYAEN)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PON1 Antibody

Western Blot: PON1 Antibody [NBP1-26401]

Western Blot: PON1 Antibody [NBP1-26401]

Western Blot: PON1 Antibody [NBP1-26401] - Analysis of PON1 in Human Liver lysate (35ug protein in RIPA buffer) using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PON1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in human liver lysates (calculated MW of 39.7 kDa band according to NP_000437.3).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PON1

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq]

Long Name

Paraoxonase 1

Alternate Names

ESA, K-45

Entrez Gene IDs

5444 (Human)

Gene Symbol

PON1

UniProt

NP_000437.3 (Human)

Additional PON1 Products

Product Documents for PON1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PON1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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