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PON3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86074

Novus Biologicals, part of Bio-Techne
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NBP1-86074
NBP1-86074-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: REVEPVEPENCHLIEELESGSEDIDILPSGLAFISSGLKYPGMPNFAPDEPGKIFLMDLNEQNPRAQALEISGGFDKELFNPHGISIFIDKDNTVYLYVVNHP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PON3 Antibody

Western Blot: PON3 Antibody [NBP1-86074]

Western Blot: PON3 Antibody [NBP1-86074]

Western Blot: PON3 Antibody [NBP1-86074] - Analysis in human cell lines A-549 and HEK293 using anti-PON3 antibody. Corresponding PON3 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunohistochemistry-Paraffin: PON3 Antibody [NBP1-86074]

Immunohistochemistry-Paraffin: PON3 Antibody [NBP1-86074]

Immunohistochemistry-Paraffin: PON3 Antibody [NBP1-86074] - Staining in human liver and kidney tissues using anti-PON3 antibody. Corresponding PON3 RNA-seq data are presented for the same tissues.
Western Blot: PON3 Antibody [NBP1-86074]

Western Blot: PON3 Antibody [NBP1-86074]

Western Blot: PON3 Antibody [NBP1-86074] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4

Applications for PON3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PON3

The PON3 gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydr

Long Name

Paraoxonase 3

Alternate Names

EC 3.1.1.2, EC 3.1.1.81, EC 3.1.8.1, paraoxanase-3, paraoxonase 3, serum paraoxonase/lactonase 3

Gene Symbol

PON3

Additional PON3 Products

Product Documents for PON3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PON3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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