POR/Cytochrome P450 Reductase Antibody (OTI3F10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47914

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F10

Concentration

1 mg/ml

View all POR/Cytochrome P450 Reductase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POR (NP_000932) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens P450 (cytochrome) oxidoreductase (POR).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

76.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POR/Cytochrome P450 Reductase Antibody (OTI3F10)

Western Blot: POR/Cytochrome P450 Reductase Antibody (OTI3F10) [NBP1-47914]

Western Blot: POR/Cytochrome P450 Reductase Antibody (OTI3F10) [NBP1-47914]

Western Blot: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 Reductase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 Reductase.
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (OTI3F10) [NBP1-47914]

Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (OTI3F10) [NBP1-47914]

Immunocytochemistry/Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 Reductase.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (OTI3F10) [NBP1-47914]

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (OTI3F10) [NBP1-47914]

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
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Applications for POR/Cytochrome P450 Reductase Antibody (OTI3F10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POR/Cytochrome P450 Reductase

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Long Name

NADPH Cytochrome P450 Reductase

Alternate Names

CPR, CYPOR, Cytochrome P450 Reductase, P450R

Entrez Gene IDs

5447 (Human)

Gene Symbol

POR

Additional POR/Cytochrome P450 Reductase Products

Product Documents for POR/Cytochrome P450 Reductase Antibody (OTI3F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POR/Cytochrome P450 Reductase Antibody (OTI3F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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