POR/Cytochrome P450 Reductase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16083

Novus Biologicals, part of Bio-Techne
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NBP2-16083
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (93%), Canine (93%), Rat (91%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POR/Cytochrome P450 Reductase Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human POR. The exact sequence is proprietary.

Reactivity Notes

Porcine (87%), Rabbit (89%). Guinea pig (89%).

Localization

Endoplasmic reticulum membrane; Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POR/Cytochrome P450 Reductase Antibody

Knockout Validated: POR/Cytochrome P450 Reductase Antibody [NBP2-16083]

Knockout Validated: POR/Cytochrome P450 Reductase Antibody [NBP2-16083]

Knockout Validated: POR/Cytochrome P450 Reductase Antibody [NBP2-16083] - Wild-type (WT) and POR knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with POR antibody [C2C3], C-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP2-16083]

Western Blot: POR/Cytochrome P450 Reductase Antibody [NBP2-16083]

Western Blot: Cytochrome P450 Reductase Antibody [NBP2-16083] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP2-16083]

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP2-16083]

Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody [NBP2-16083] - hepatoma. POR antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for POR/Cytochrome P450 Reductase Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POR/Cytochrome P450 Reductase

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq]

Long Name

NADPH Cytochrome P450 Reductase

Alternate Names

CPR, CYPOR, Cytochrome P450 Reductase, P450R

Gene Symbol

POR

UniProt

P16435

Additional POR/Cytochrome P450 Reductase Products

Product Documents for POR/Cytochrome P450 Reductase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POR/Cytochrome P450 Reductase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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