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Porcine/Equine CD14 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4597G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Porcine, Equine

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 433423

Product Specifications

Immunogen

E. coli-derived recombinant porcine CD14

Specificity

Detects porcine CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD14 and recombinant mouse CD14 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Porcine/Equine CD14 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Porcine peripheral blood mononuclear cells (untreated) and equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD14

CD14 is a 55 kDa GPI-linked cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. It is a pattern recognition protein that acts as a receptor for lipopolysaccharide (LPS) and other microbial cell wall components. CD14 also occurs as a secreted protein. Mature porcine CD14 shares 70‑75% aa identity with human, cow, sheep, water buffalo and horse CD14 and 61‑62% aa identity with mouse and rat CD14.

Alternate Names

CD14

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Gene Symbol

CD14

Additional CD14 Products

Product Documents for Porcine/Equine CD14 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Porcine/Equine CD14 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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