Porcine IL-1ra/IL-1F3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF780
Key Product Details
Species Reactivity
Validated:
Porcine
Cited:
Porcine
Applications
Validated:
Immunocytochemistry, Neutralization, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant porcine IL‑1ra/IL‑1F3
His26-Gln177
Accession # Q29056
His26-Gln177
Accession # Q29056
Specificity
Detects porcine IL‑1ra/IL‑1F3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Porcine IL-1ra/IL-1F3 Antibody
IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha/IL‑1F1-dependent Cell Proliferation and Neutral-ization by Porcine IL‑1ra/ IL‑1F3 Antibody.
Recombinant Porcine IL-1ra/ IL-1F3 (Catalog # 780-RA) inhibits Recombinant Porcine IL-1a/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1a/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.2 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND50 is typically 3-15 µg/mL.
IL‑1ra/IL‑1F3 in Porcine PBMCs.
IL-1ra/IL-1F3 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Porcine IL-1ra/ IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF780) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Porcine IL-1ra/IL-1F3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed porcine peripheral blood mononuclear cells
Sample: Immersion fixed porcine peripheral blood mononuclear cells
Western Blot
0.1 µg/mL
Sample: Recombinant Porcine IL-1ra/IL-1F3 (Catalog # 780-RA)
Sample: Recombinant Porcine IL-1ra/IL-1F3 (Catalog # 780-RA)
Neutralization
Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha/IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J. A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 0.2 µg/mL Recombinant Porcine IL‑1ra/IL‑1F3 and 75 pg/mL Recombinant Porcine IL‑1 alpha/IL‑1F1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1ra/IL-1F3
Long Name
Interleukin 1 Receptor Antagonist
Alternate Names
DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4
Gene Symbol
IL1RN
UniProt
Additional IL-1ra/IL-1F3 Products
Product Documents for Porcine IL-1ra/IL-1F3 Antibody
Product Specific Notices for Porcine IL-1ra/IL-1F3 Antibody
For research use only
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