Porcine IL-2 Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC6521V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
Intracellular Staining by Flow Cytometry
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 100312
Product Specifications
Immunogen
E. coli-derived recombinant porcine IL-2
Ala21-Thr154
Accession # P26891
Ala21-Thr154
Accession # P26891
Specificity
Detects porcine IL-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with human, mouse, rat, bovine, canine, equine, feline, or cotton rat IL-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Porcine IL-2 Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Sample: Porcine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Sample: Porcine peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that in addition to its T cell growth factor activity, IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mature porcine and human IL-2 share approximately 72% amino acid sequence identity.The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits. The intermediate-affinity IL-2 receptor complex, which lacks the alpha subunit, but contains both the beta and gamma subunits, is also capable of transducing the IL-2 signal. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma chain of the IL-2 receptor complex is also a subunit of IL-4, IL-7, and IL-9 receptor complexes.
References
- Taniguchi, T. and Y. Minami (1993) Cell 73:5.
- Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
- Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
UniProt
Additional IL-2 Products
Product Documents for Porcine IL-2 Alexa Fluor® 405-conjugated Antibody
Product Specific Notices for Porcine IL-2 Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...