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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # OCT2/2137

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human POU2F2 protein (around aa 112-297) (exact sequence is proprietary) (Uniprot: P09086)

Localization

Nuclear

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75734).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for POU2F2 Antibody (OCT2/2137) - Azide and BSA Free

Western Blot: POU2F2 Antibody (OCT2/2137)Azide and BSA Free [NBP2-75735]

Western Blot: POU2F2 Antibody (OCT2/2137)Azide and BSA Free [NBP2-75735]

Western Blot: POU2F2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Western Blot Analysis (A) Recombinant Protein (B) Daudi cell lysate POU2F2 Mouse Monoclonal Antibody (OCT2/2137).
Immunohistochemistry-Paraffin: POU2F2 Antibody (OCT2/2137) - Azide and BSA Free [NBP2-75735]

Immunohistochemistry-Paraffin: POU2F2 Antibody (OCT2/2137) - Azide and BSA Free [NBP2-75735]

Immunohistochemistry-Paraffin: POU2F2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Formalin-fixed, paraffin-embedded human Tonsil stained with POU2F2 Mouse Monoclonal Antibody (OCT2/2137).
Western Blot: POU2F2 Antibody (OCT2/2137)Azide and BSA Free [NBP2-75735]

Western Blot: POU2F2 Antibody (OCT2/2137)Azide and BSA Free [NBP2-75735]

Western Blot: POU2F2 Antibody (Oct2/2137) - Azide and BSA Free [NBP2-75735] - Western Blot Analysis Ramos cell lysate using POU2F2 Mouse Monoclonal Antibody (OCT2/2137).

Applications for POU2F2 Antibody (OCT2/2137) - Azide and BSA Free

Application
Recommended Usage

ELISA

1 - 2 ug/mL

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1 - 2 ug/mL

Western Blot

2 ug/mL
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: POU2F2

Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5'- ATTTCAT-3'). Mostly expressed in B cells, Oct-2 posses a "leucine zipper" domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.

Alternate Names

homeobox protein, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, OCT2, Octamer-binding protein 2, Octamer-binding transcription factor 2, OTF2, OTF-2, POU class 2 homeobox 2, POU domain, class 2, transcription factor 2

Gene Symbol

POU2F2

UniProt

Additional POU2F2 Products

Product Documents for POU2F2 Antibody (OCT2/2137) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POU2F2 Antibody (OCT2/2137) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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