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POU2F2 Antibody (OCT2/7073R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13743

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # OCT2/7073R

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant fragment of human POU2F2 protein (around aa 112-297) (exact sequence is proprietary) (Uniprot: P09086)

Localization

Nucleus

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14146)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for POU2F2 Antibody (OCT2/7073R)

Immunohistochemistry-Paraffin: POU2F2 Antibody (OCT2/7073R) [NBP3-13743]

Immunohistochemistry-Paraffin: POU2F2 Antibody (OCT2/7073R) [NBP3-13743]

Immunohistochemistry-Paraffin: POU2F2 Antibody (OCT2/7073R) [NBP3-13743] - Formalin-fixed, paraffin-embedded human tonsil stained with POU2F2 antibody (OCT2/7073R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
OCT2 Antibody (OCT2/7073R)

OCT2 Antibody (OCT2/7073R)

Formalin-fixed, paraffin-embedded human tonsil stained with OCT2 antibody (OCT2/7073R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for POU2F2 Antibody (OCT2/7073R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: POU2F2

Octamer transcription factor-2 (Oct-2) is a transcription factor that specifically binds to the octamer motif (5'- ATTTCAT-3'). Mostly expressed in B cells, Oct-2 posses a "leucine zipper" domain and belongs to the POU family of transcription factors (1-3). Additionally, Oct-2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression.

Alternate Names

homeobox protein, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, OCT2, Octamer-binding protein 2, Octamer-binding transcription factor 2, OTF2, OTF-2, POU class 2 homeobox 2, POU domain, class 2, transcription factor 2

Gene Symbol

POU2F2

UniProt

Additional POU2F2 Products

Product Documents for POU2F2 Antibody (OCT2/7073R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POU2F2 Antibody (OCT2/7073R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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