POU3F2/OCT7 Antibody (CL6232)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61437
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL6232
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLVQGDYGALQSNGHP
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for POU3F2/OCT7 Antibody (CL6232)
Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437]
Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of U-251 cells using the Anti-POU3F2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437]
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of rat cerebral cortex shows absence of positivity in layer 6 neurons as expected.Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437]
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody (CL6232) [NBP2-61437] - Staining of mouse forebrain shows nuclear immunoreactivity in layers 2-5 of cerebral cortex.Applications for POU3F2/OCT7 Antibody (CL6232)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POU3F2
Long Name
POU Class 3 Homeobox 2
Alternate Names
Brain-2, BRN2, Oct7, OTF7
Gene Symbol
POU3F2
Additional POU3F2 Products
Product Documents for POU3F2/OCT7 Antibody (CL6232)
Product Specific Notices for POU3F2/OCT7 Antibody (CL6232)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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