POU3F2/OCT7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21585

Novus Biologicals, part of Bio-Techne
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NBP2-21585
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chimpanzee

Cited:

Mouse

Predicted:

Bovine (100%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POU3F2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human POU3F2/OCT7. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POU3F2/OCT7 Antibody

Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585]

Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585]

Immunocytochemistry/Immunofluorescence: POU3F2/OCT7 Antibody [NBP2-21585] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Scale bar = 10 um.
Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - Rat brain. Brn2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of OCT7 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
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Applications for POU3F2/OCT7 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay-dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POU3F2

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Long Name

POU Class 3 Homeobox 2

Alternate Names

Brain-2, BRN2, Oct7, OTF7

Gene Symbol

POU3F2

UniProt

P20265

Additional POU3F2 Products

Product Documents for POU3F2/OCT7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POU3F2/OCT7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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