POU4F3 Antibody (5B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005459-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all POU4F3 Antibodies »

Product Specifications

Immunogen

POU4F3 (NP_002691, 100 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDPRELEAF

Specificity

POU4F3 - POU domain, class 4, transcription factor 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for POU4F3 Antibody (5B8)

Western Blot: POU4F3 Antibody (5B8) [H00005459-M01]

Western Blot: POU4F3 Antibody (5B8) [H00005459-M01]

Western Blot: POU4F3 Antibody (5B8) [H00005459-M01] - Analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: POU4F3 Antibody (5B8) [H00005459-M01]

Immunocytochemistry/ Immunofluorescence: POU4F3 Antibody (5B8) [H00005459-M01]

Immunocytochemistry/Immunofluorescence: POU4F3 Antibody (5B8) [H00005459-M01] - Analysis of monoclonal antibody to POU4F3 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: POU4F3 Antibody (5B8) [H00005459-M01]

Western Blot: POU4F3 Antibody (5B8) [H00005459-M01]

Western Blot: POU4F3 Antibody (5B8) [H00005459-M01] - Analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi ( Cat # H00005459-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 (Cat # H00005459-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for POU4F3 Antibody (5B8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: POU4F3

The POU4F3 gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determi

Alternate Names

brain-3C, Brain-specific homeobox/POU domain protein 3C, BRN3.1, Brn-3C, BRN3Cbrn-3C, DFNA15, MGC138412, POU class 4 homeobox 3, POU domain class 4, transcription factor 3, POU domain, class 4, transcription factor 3

Entrez Gene IDs

5459 (Human)

Gene Symbol

POU4F3

OMIM

602 (Human)

UniProt

NP_002691 (Human)

Additional POU4F3 Products

Product Documents for POU4F3 Antibody (5B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POU4F3 Antibody (5B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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