Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G3

Format

BSA Free

Concentration

1.0 mg/ml

View all PP1 alpha Antibodies »

Product Specifications

Immunogen

Recombinant human PP1 alpha/PPP1A (30-299aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PP1 alpha/PPP1A Antibody (4G3) - BSA Free

Western Blot: PP1 alpha/PPP1A Antibody (4G3)BSA Free [NBP1-04334]

Western Blot: PP1 alpha/PPP1A Antibody (4G3)BSA Free [NBP1-04334]

Western Blot: PP1 alpha/PPP1A Antibody (4G3) [NBP1-04334] - Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: PP1 alpha/PPP1A Antibody (4G3) - BSA Free [NBP1-04334]

Immunocytochemistry/ Immunofluorescence: PP1 alpha/PPP1A Antibody (4G3) - BSA Free [NBP1-04334]

Immunocytochemistry/Immunofluorescence: PP1 alpha/PPP1A Antibody (4G3) [NBP1-04334] - Analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for PP1 alpha/PPP1A Antibody (4G3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP1 alpha

Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase 2. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis

Long Name

Protein Phosphatase 1 Catalytic Subunit alpha

Alternate Names

PPP1A, PPP1CA

Gene Symbol

PPP1CA

UniProt

NP_002699 (Human)

Additional PP1 alpha Products

Product Documents for PP1 alpha/PPP1A Antibody (4G3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PP1 alpha/PPP1A Antibody (4G3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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