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PP2A alpha Antibody (HL1447) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25611

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25611

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Drosophila, Feline

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rabbit (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1447

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2A alpha Antibody (HL1447) - Azide and BSA Free

PP2A alpha Antibody (HL1447) - Azide and BSA Free

Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] -

Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PP2A alpha Antibody (HL1447) - Azide and BSA Free

Knockdown Validated: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] -

Knockdown Validated: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - Non-transfected (-) and transfected (+) PP2A alpha shRNA-transfected 293T (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PP2A alpha Antibody (HL1447) - Azide and BSA Free

Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] -

Immunohistochemistry-Paraffin: PP2A alpha Antibody (HL1447) - Azide and BSA Free [NBP3-25611] - PP2A alpha antibody [HL1447] detects PP2A alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. PP2A alpha stained by PP2A alpha antibody [HL1447] (NBP3-25611) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for PP2A alpha Antibody (HL1447) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2A

Protein phosphatase type 2A (PP2A ) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways. PP2A is comprised of a core enzyme (which is made up of catalytic C and regulatory A (PR65) subunits) and a secondary regulatory B subunit. PP2A alpha refers to the alpha isoform of the PP2A catalytic subunit.

Alternate Names

PP2CA, PPP2CA, RP-C

Gene Symbol

PPP2CA

Additional PP2A Products

Product Documents for PP2A alpha Antibody (HL1447) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP2A alpha Antibody (HL1447) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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