Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PP2A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 260 and the C-terminus (residue 309) of human Protein Phosphatase 2A using the numbering given in entry NP_004147.1 (GeneID 5516). The region is identical to that of PPP2CA (NP_00

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2A alpha Antibody - BSA Free

Western Blot: PP2A alpha Antibody [NB100-2260]

Western Blot: PP2A alpha Antibody [NB100-2260]

Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PP2A alpha Antibody [NB100-2260]

Immunoprecipitation: PP2A alpha Antibody [NB100-2260]

Immunoprecipitation: PP2A alpha Antibody [NB100-2260] - Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 (lot 2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-PP2A antibody NB100-2259. For blotting immunoprecipitated PP2A, NB100-2260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PP2A alpha Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PP2A

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in the 5' UTR of this gene may cause a rare form of autosomal dominant spinocerebellar ataxia 12.

Alternate Names

PP2CA, PPP2CA, RP-C

Entrez Gene IDs

5515 (Human); 19052 (Mouse)

Gene Symbol

PPP2CA

Additional PP2A Products

Product Documents for PP2A alpha Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP2A alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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