PP2C alpha/PPM1A Antibody (p6c7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04333
![Western Blot: PP2C alpha/PPM1A Antibody (p6c7)BSA Free [NBP1-04333]](https://resources.bio-techne.com/images/products/PP2C-alpha-PPM1A-Antibody-p6c7-Western-Blot-NBP1-04333-img0003.jpg "Western Blot: PP2C alpha/PPM1A Antibody (p6c7)BSA Free [NBP1-04333]")
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # p6c7
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human PP2C alpha/PPM1A (1-382aa) purified from E. coli (NP_066283).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PP2C alpha/PPM1A Antibody (p6c7) - BSA Free
Western Blot: PP2C alpha/PPM1A Antibody (p6c7)BSA Free [NBP1-04333]
Western Blot: PP2C alpha/PPM1A Antibody (p6c7) [NBP1-04333] - Effect of NOX1 knockdown on phosphatase activity and expression in HT-29 cells. Western analysis demonstrates the protein expression and phosphorylation status of several members of the protein-tyrosine and serine/threonine phosphatase families, respectively, in HT-29, SC, and 6A cells. As described under -Experimental procedures," the band intensities have been quantitated; comparisons were made between parental HT-29 cells, the SC scrambled controls, and the 6A knockdown clones. Each intensity calculation is the mean +/- S.D. of 2-7 individual experiments. Image collected and cropped by CiteAb from the following publication (https://www.jbc.org/lookup/doi/10.1074/jbc.M116.768283) licensed under a CC-BY license.![Western Blot: PP2C alpha/PPM1A Antibody (p6c7)BSA Free [NBP1-04333]](https://resources.bio-techne.com/images/products/PP2C-alpha-PPM1A-Antibody-p6c7-Western-Blot-NBP1-04333-img0002.jpg "Western Blot: PP2C alpha/PPM1A Antibody (p6c7)BSA Free [NBP1-04333]")
Western Blot: PP2C alpha/PPM1A Antibody (p6c7)BSA Free [NBP1-04333]
Western Blot: PP2C alpha/PPM1A Antibody (p6c7) [NBP1-04333] - Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2Calpha(1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates PP2Calpha(~43kDa).Applications for PP2C alpha/PPM1A Antibody (p6c7) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:250-1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PP2C alpha/PPM1A
Long Name
Protein Phosphatase 2C alpha
Alternate Names
MMPa-2, MPPa-1, Pp2c1, PPM1A
Gene Symbol
PPM1A
UniProt
Additional PP2C alpha/PPM1A Products
Product Documents for PP2C alpha/PPM1A Antibody (p6c7) - BSA Free
Product Specific Notices for PP2C alpha/PPM1A Antibody (p6c7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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