PP2C alpha/PPM1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13796

Novus Biologicals, part of Bio-Techne
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NBP2-13796
NBP2-13796-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PP2C alpha/PPM1A Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: GQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSGAPHR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2C alpha/PPM1A Antibody

Western Blot: PP2C alpha/PPM1A Antibody [NBP2-13796]

Western Blot: PP2C alpha/PPM1A Antibody [NBP2-13796]

Western Blot: PP2C alpha/PPM1A Antibody [NBP2-13796] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-441
Immunocytochemistry/ Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP2-13796]

Immunocytochemistry/ Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP2-13796]

Immunocytochemistry/Immunofluorescence: PP2C alpha/PPM1A Antibody [NBP2-13796] - Staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP2-13796]

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP2-13796]

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody [NBP2-13796] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Applications for PP2C alpha/PPM1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C alpha/PPM1A

Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C

Long Name

Protein Phosphatase 2C alpha

Alternate Names

MMPa-2, MPPa-1, Pp2c1, PPM1A

Gene Symbol

PPM1A

Additional PP2C alpha/PPM1A Products

Product Documents for PP2C alpha/PPM1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PP2C alpha/PPM1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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