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PP2C epsilon/PPM1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87248

Novus Biologicals, part of Bio-Techne
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NBP1-87248

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLCDKDGNAIPLSHDHKPYQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLNVVIPDPDILTF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PP2C epsilon/PPM1L Antibody

Western Blot: PP2C epsilon/PPM1L Antibody [NBP1-87248]

Western Blot: PP2C epsilon/PPM1L Antibody [NBP1-87248]

Western Blot: PP2C epsilon/PPM1L Antibody [NBP1-87248] - Analysis in control (vector only transfected HEK293T lysate) and PPM1L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PP2C epsilon/PPM1L Antibody [NBP1-87248]

Immunohistochemistry-Paraffin: PP2C epsilon/PPM1L Antibody [NBP1-87248]

Immunohistochemistry-Paraffin: PP2C epsilon/PPM1L Antibody [NBP1-87248] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Applications for PP2C epsilon/PPM1L Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C epsilon/PPM1L

PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al., 2004 [PubMed 15560375]).[supplied by OMIM]

Long Name

Protein Phosphatase 2C alpha

Alternate Names

PP2CE, PPM1L

Gene Symbol

PPM1L

Additional PP2C epsilon/PPM1L Products

Product Documents for PP2C epsilon/PPM1L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PP2C epsilon/PPM1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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