PP2C gamma/PPM1G Antibody (112)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89858

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 112

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PP2C gamma Antibodies »

Product Summary for PP2C gamma/PPM1G Antibody (112)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human PP2C gamma/PPM1G (Uniprot#: O15355; Met317-Asp546).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PP2C gamma/PPM1G Antibody (112)

Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858]

Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858]

Western Blot: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Anti-PP2C gamma/PPM1g rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:59 kDa.
Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (112) [NBP2-89858]

Immunocytochemistry/ Immunofluorescence: PP2C gamma/PPM1G Antibody (112) [NBP2-89858]

Immunocytochemistry/Immunofluorescence: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of Human PPM1G in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PPM1G monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858]

Immunohistochemistry-Paraffin: PP2C gamma/PPM1G Antibody (112) [NBP2-89858] - Staining of human PPM1G in human epididymis with rabbit monoclonal antibody (1:200).
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Applications for PP2C gamma/PPM1G Antibody (112)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

Additional PP2C gamma Products

Product Documents for PP2C gamma/PPM1G Antibody (112)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PP2C gamma/PPM1G Antibody (112)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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